Western blot analysis of PLCD4 in extracts of various cell lines. Samples were incubated with PLCD4 Polyclonal antibody (Product # PA5-110179) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Immunocytochemistry analysis of PLCD4 in U-2OS cells. Samples were incubated in polyclonal PLCD4 antibody (Product # PA5-110179) using a dilution of 1:100, followed by DAPI.
Supplier Page from Thermo Fisher Scientific for PLCD4 Antibody